CDSN (Corneodesmosin; also S protein) is a 52-56kD, secreted glycoprotein that has an unusually high content of Gly, Pro and Ser.
It is found in the desmosomes of spinous layer keratinocytes, and presumably uses its high Gly content to generate homophilic noncovalent intercellular bridges.
Mature human CDSN is 497 amino acids (aa) in length.
It contains a Ser-rich region (aa62-464) and one Gly-rich domain (aa60-171).
CDSN undergoes sequential proteolytic processing to generate multiple fragments that vary in size from 46-43kD, to 5-30kD, to 15kD.
This sequential extracellular processing allows initially for CDSN incorporation into a functional adhesional junction, and later for the removal of adhesional glycine loops with a subsequent dissociation (desquamation) of cells.
There is one potential isoform termed the S protein that shows a two aa substitution for the C-terminal 29 amino acids.
Over aa 33-529, human CDSN shares 68% aa identity with mouse CDSN.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/mlWestern Blot:1ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a powder lyophilized in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human CDSN
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：NS0-derived recombinant human CDSN corresponding to Lys33-Pro529 (Ser153 del)
