Complexin-2 (CPX II; also known as synaphin-1) is a 19kD, cytosolic protein that belongs to the complexin/synaphin family of molecules.
It is expressed in excitatory neurons and serves as a block against premature granule release by its binding to the cis-SNARE complex that contains SNAP25, VAMP2 and STX1A.
Human Complexin-2 is 134 amino acids (aa) in length and contains one coiled-coil domain (aa 28-84) that incorporates part of a SNARE complex interaction sequence (aa 41-97).
There is one potential splice variant that shows a premature truncation after Glu114.
Human Complexin-2 is identical in aa sequence to that for mouse and rat Complexin-2.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml.
Lysates from human, mouse and rat brain tissue were resolved by SDS-PAGE, transferred to Immobilon-P membrane and immunoblotted with 1ug/ml goat anti-Complexin-2.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute in PBS containing 0.02% sodium azide.
Specificity：Detects endogenous human, mouse and rat Complexin-2 at 19kD using Western blot.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Purified, E. coli-derived, recombinant human Complexin-2 (rhCPX II; aa 1-134; UniProt # Q6PUV4).
