84-1111-73　Clostridium botulinum Neurotoxin Type C1 Heavy Chain (BoNT-C1) 100ug　C5851-49B

特徴

• BoNT-C1 (Botulinum Neurotoxin Type C1 [Heavy Chain]) is a 100kD C-terminal fragment of the C1 Clostridum botulinum neurotoxin (holo)precursor.
• It constitutes a nonenzymatic part of a Zn 2+ -dependent M27 family endoprotease that potently blocks neurosecretion.
• The inactive 1291aa, 150kD C1 precursor is cleaved between Lys449Thr450 to generate a heterodimeric toxin held together by a preexisting interchain disulfide bond between Cys437 and Cys453.
• The 100kD C-terminus (heavy chain) acts as a ligand for neuronal membrane ecto-acceptors, its inducing internalization with translocation.
• The 50kD N-terminal light chain acts as an intracellular peptidase that cleaves SNAP-25 and syntaxin.
• This is suggested to block noradrenalin release from a readily releasable pool of vesicles.
• Over aa866-1291, the C1 heavy chain shares less than 40% aa identity with the D-type neurotoxin heavy chain.
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.1ug/ml with BoNT-C1 Heavy Chain Receptor Binding DomainImmunoprecipitation: 25ug/ml immunoprecipitates BoNT-C1 heavy chain spiked in E.
• coli cell lysate
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.