Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120-160kD member of the neuregulin family of proteins.
Depending upon its expression profile, it may be a glycoprotein oft 120kD, or a chondroitin sulfate (CS) proteoglycan of 140-160kD.
NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons.
Mature human NGC is a 536 amino acid (aa) type I transmembrane glycoprotein.
It possesses a 393 aa extracellular region (aa 31-423) that contains a Ser117 CS attachment site and an EGF-like domain aa 371-413).
There are at least two isoform variants.
One shows a deletion of aa87-513, while a second shows an alternative start site at Met139 with the same deletion.
Phosphorylation likely occurs on Ser249, and proteolysis generates a > 75kD soluble fragment.
Over aa 31-420, human NGC shares 84% aa identity with mouse NGC.
Applications:Suitable for Immunohistochemistry ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlImmunohistochemistry: 10ug/ml detects Neuroglycan C in sections of adult mouse cerebellum.
Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT After blocking, cells were incubated with diluted primary antibody for 3 hours at RTfollowed by donkey anti-goat lgG at RT for 1 hour.
Between each step, cells were washed with PBS containing 0.1% BSA.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by mouse Neuroglycan C affinity chromatography.
Form：Lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.5ml of PBS is used, the antibody concentration will be 0.2mg/ml.
Specificity：Recognizes Neuroglycan C, extracellular domain. In direct ELISA, this antibody shows 25% cross-reactivity with rhNeuroglycan C. Species Crossreactivity: Mouse, rat.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Mo Rt
Immunogen：purified, CHO cell-derived, recombinant mouse Neuroglycan C (rmNeuroglycan C; aa 31-420; Accession# AAH55736).
