Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120-160kD member of the neuregulin family of proteins.
Depending upon its expression profile, it may be a glycoprotein of 120kD, or a chondroitin sulfate (CS) proteoglycan of 140-160kD.
NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons.
Mature human NGC is a 536 amino acid (aa) type I transmembrane glycoprotein.
It possesses a 393 aa extracellular region (aa31-423) that contains a Ser117 CS attachment site and an EGF-like domain (aa 371-413).
There are at least two isoform variants.
One shows a deletion of aa 487-513, while a second shows an alternaive start site at Met139 with the same deletion.
Phosphorylation likely occurs on Ser249, and proteolysis generates a >75kD soluble fragment.
Over aa 31-420, human NGC shares 84% aa identity with mouse NGC.
Applications:Suitable for use in ELISA, Immunocytochemistry and Western Blot.
Other Applications not tested.
Recommended Dilution:Western Blot: 1ug/ml SH-SY5Y human neuroblastoma cellImmunocytochemistry: 1-15ug/ml Perfusion frozen sections of mouse brain (cerebellum)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Neuroglycan C. Shows approximately 50% cross-reactivity with recombinant mouse Neuroglycan C in direct ELISA.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Val31-Gln420 of human Neuroglycan C extracellular domain, expressed in CHO cells.
