Integrin alpha 6 (also VLA-6, CD49F and ITA6) is a 130-150kD member of the Integrin alpha chain family of molecules.
Mature Integrin alpha 6 is a 1107aa type I transmembrane glycoprotein that contains a 1027aa extracellular region and a 93 aa cytoplasmic tail.
The ECD is cleaved to generate a disulfide-linked dimer that shows a 125kD heavy chain (aa24-938), and a 30-31kD membrane-bound light chain (aa942-1130).
Integrin alpha 6 forms a nondisulfide-linked heterodimeric complex with both Integrin beta1 and beta4.
Each complex serves as a receptor for laminin.
Splice variants exist that show either a deletion of aa215-258 or a deletion of aa260-298, possibly accompanied by a 29aa substitution for aa1084-1130.
Over aa93-148 and 518-574, human Integrin alpha6 shares 89% aa identity with mouse Integrin alpha6.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1.0ug/mlWestern Blot: 1.0ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity：Recognizes human Integrin alpha 6.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human Integrin alpha 6 aa93-148; aa55-613 (E. coli).
