4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily.
It is alternatively known as TNFRSF9, CD137, and ILA.
The human and mouse proteins share 60% amino acid sequence identity.
4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation.
Applications:Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 2.5ug/10e6 cells using PHA-treated human T cellsELISA (Capture): 0.2-0.8ug/mlELISA (Detection): 0.1-0.4ug/mlWestern Blot: 0.1ug/ml Immunocytochemistry: 5-15ug/ml using immersion fixed human peripheral blood mononuclear cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Reconstitute with 250ul sterile PBS.
Specificity：Recognizes human CD137. Species Crossreactivity: rmCD137, rhNGF R, rhTNF RI, rhTNF RII; <0.1%
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa24-186 from human CD137, expressed in NS0 cells (Q07011).
