CD137 is an inducible T cell surface protein belonging to the TNF receptor superfamily.
The 255aa human CD137 is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily.
The 30kD glycoprotein exists both as a monomer and as a dimer on T cells.
The human and mouse proteins share 60% aa identity.
CD137 is absent from naive T cells, but it is upregulated and continually expressed following T cell activation.
The natural ligand, CD137L, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells.
Cross-linking of CD137 by CD137L or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies.
CD137 signals through the TFAF2-NIK pathway resulting in activation of NF-kappaB and ultimately promoting proliferation and survival of T cells
Applications:Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunocytochemistry and Co-stimulation of T cells.
Other applications not tested
Recommended Dilutions:Flow Cytometry: 25ug/ml.
10ul of diluted C2548-40E labels 1-2.5x10e5 cells in a total reaction volume ≤200ul.
Validated using PHA-treated T cells
ELISA: 0.2-0.8ug/ml as a capture antibody in a sandwich ELISA in combination with biotinylated human CD137 detection antibody (C2548-40F) and recombinant human CD137 as the standard.
Western Blot: 0.1- 0.2ug/ml.
The detection limit for recombinant human CD137 is ~0.2ng/lane and 0.5ng/lane under non-reducing and reducing conditions, respectively
Immunocytochemistry: 2-15ug/ml will detect CD137 in cells
Co-stimulation of T cells: 5-20ug/ml stimulates IFN-gamma production of human T cells in the presence of 10ug/ml of monoclonal anti-human CD3
Optimal dilutions to be determined by the researcher
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Specificity：Recognizes the extracellular domain of human CD137. In ELISA, <0.1% crossreactivity with recombinant mouse CD137, recombinant human NGF R, TNF RI and TNF RII is observed. In Western Blot, shows <1% crossreactivity with recombinant human NGF R, sTNF RI, sTNF RII and recombinant mouse sTNF RI and sTNF RII.
Isotype：IgG
Calc Applications Abbrev：E FC IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to the extracellular domain of human CD137 expressed in NSO cells.
