CD55 (Decay-accelarating factor/DAF) is a glycoprotein member of the RCA family of molecules.
It is found on blood cells, epithelium and endothelium and serves both as a receptor for CD97 and a negative regulator of the C3 convertases, C4b2a and C3bBb.
Mature mouse CD55 is the product of two genes that arose by duplication.
There is a 55-60kD, 356 amino acid (aa), GPI-linked form that is ubiquitously expressed.
This molecule contains four SUSHI domains (aa 35-285), a Ser/Thr-rich region (aa 288-362) and a GPI-anchor at Gly362.
There is also a 50kD, 379 aa, type I transmembrane form that is testis-associated.
It shows the same domain architecture and is 93% aa identical to the GPI-form.
At least four GPI gene isoforms exist.
They diverge after Ile285 and show deletions and substitutions.
Over aa 35-359, mouse CD55 is 66% and 50% aa identical to rat and human CD55, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse CD55.
The detection limit for rmCD55 is approximately 1ng/lane and 5ng/lane under non-reducing and reducing conditions, respectively
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS containing 50ug of BSA. Reconstitute with sterile Tris-buffered saline pH 7.3 (20mM Tris base, 150mM NaCl) containing 0.1% BSA. If 1ml of buffer is used, the antibody concentration will be 50ug/ml.
Specificity：Recognizes mouse CD55. Shows less than 1% cross-reactivity with rhCD55 and rmCD97.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Purified, NS0-derived, recombinant mouse CD55 (rmCD55; aa 35-359; Accession# Q61475).
