Integrin alpha 1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190-210kD member of the integrin alpha chain family of molecules.
It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium.
Integrin alpha 1 forms a noncovalent heterodimer with Integrin beta 1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI.
It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus.
Mature human Integrin alpha 1 is a 1151 aa type I transmembrane glycoprotein that contains a 1113aa extracellular domain (ECD) and a 15aa cytoplasmic tail.
The ECD contains one vWFA/I-domain (aa147-360) that binds collagen, plus multiple divalent cation binding sites.
Potential splice variants exist that show a two and four aa substitution for aa 765-1179.
Over aa29-1141 (the ECD), human Integrin alpha 1 shares 88% aa identity with mouse Integrin alpha 1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1.0ug/mlWestern Blot: 1.0ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile 40-50% glycerol, PBS..
Aliquot and store at -20°C.
Reconstituted product is stable for 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity：Recognizes human Integrin alpha 1 extracellular domain.
