CHST5 (Carbohydrate sulfotransferase 5; also GlcNAc6ST-3, I-GlcNAc6ST and GST4 alpha) is likely a 53-56kD member of the sulfotransfease family of enzymes.
It is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium.
It appears to act only on short carbohydrates and transfers sulfate to position #6 of mucin-associated GlcNAc.
Human CHST5 is a 390aa type II transmembrane protein.
It contains a short cytoplasmic segment (aa1-9) and a long luminal domain (aa28-390) that contains an enzymatic region (aa180-358).
There is one alternate start site that is 22aa upstream of the standard site.
Humans have a GST4-alpha and -beta gene, the result of gene duplication.
Over aa28-390, these two gene products share 87% aa identity.
Mouse has only one GST4 gene, and it is functionally related to human GST4- beta.
Over aa 28-390, human GST4-alpha/CHST5 is 78% aa identical to mouse GST4.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1.0ug/mlWestern Blot: 1.0ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile 40-50% glycerol, PBS..
Aliquot and store at -20°C.
Reconstituted product is stable for 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity：Recognizes human CHST5.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：CHO cell-derived, recombinant human CHST5 lumenal domain aa28-390; Q9GZS9.
