C4.4A (Ly6/PLAUR-domain containing protein 3; also MIG-C4 and GPI-anchored metastasis-associated protein C4.4A) is an 85-95kD, variably glycosylated member of the Ly6 family of GPI-anchored proteins.
It is expressed on transitional epithelium, keratinocytes and monocytes, and binds laminin 1 and 5, plus Galectin-3.
C4.4A is believed to support cell migration.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applicatons not tested.
Recommended Dilutions:Western Blot: 1ug/ml.
Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted
Immunohistochemistry: 2-15ug/ml will detect C4.4A in cells and tissues
Optimal dilution determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse C4.4A. In direct ELISA, this antibody shows approximately 40% cross-reactivity with rhC4.4A/LYPD3.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Leu33-His287 from mouse C4.4A expressed in NS0 cells (Q91YK8).
