LYPD3 (Ly6/PLAUR-domain containing protein 3; also MIG-C4 and GPI-anchored metastasis-associated protein C4.4A) is an 85-95kD, variably glycosylated member of the Ly6 family of GPI-anchored proteins.
It is expressed on transitional epithelium, keratinocytes and monocytes, and binds laminin 1 and 5, plus Galectin-3.
LYPD3 is believed to support cell migration.
Mature human LYPD3 is a 296aa GPI-linked glycoprotein.
It contains two UPAR domains (aa33-126 and 140-222), and an ADAM cleavage site (aa312-325).
The molecule shows extensive N- and O-linked glycosylation.
There is one potential isoform that shows a deletion of aa129-180.
Over aa 31-286, human LYPD3 shares 81% aa identity with mouse LYPD3.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml.
Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-hC4.4A.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human C4.4A. In direct ELISA, shows ~15% cross-reactivity with rmC4.4A.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu31-His286 from human C4.4A expressed in NSO cells (O95274).
