BACH1 (BTB and CNC homolog 1; also basic leucine zipper transcription factor 1) is a ubiquitously expressed member of the Bach family of transcription factors.
Although its predicted MW is 82kD, it runs anomalously at 110kD in SDS-PAGE.
BACH1 forms noncovalent homodimers, and heterodimers with Maf oncoproteins and p53-related proteins.
It apparently serves as an architectural component for gene regulatory protein complexes.
Mouse BACH1 is 739 amino acids (aa) in length.
It contains a protein-interaction BTB domain (aa24-127), a DNA-binding motif (aa565-580), and a Leu-zipper domain (aa588-610).
This molecule should not be confused with Bach1/Fancj/Brip1 helicase.
Over aa133-513, mouse BACH1 shares 75% and 88% aa identity with human and rat BACH1, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml on NIH-3T3 mouse embryonic fibroblast cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse BACH1 at 82kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser133-Gly513 from mouse BACH1, expressed in E. coli.
