84-1111-47　BTB and CNC homology 1(BACH1, basic leucine zipper transcription factor 1, HA2303, Transcription regulator protein BACH1) 100ug　B4999-02

特徴

• BACH1 (BTB and CNC homolog 1; also basic leucine zipper transcription factor 1) is a ubiquitously expressed member of the Bach family of transcription factors.
• Although its predicted MW is 82kD, it runs anomalously at 110kD in SDS-PAGE.
• BACH1 forms noncovalent homodimers, and heterodimers with Maf oncoproteins and p53-related proteins.
• It apparently serves as an architectural component for gene regulatory protein complexes.
• Human BACH1 is 736 aa in length.
• It contains a protein-interaction BTB domain (aa 24-127), a DNA-binding motif (aa 562-577), and a Leu-zipper domain (aa 585-607).
• This molecule should not be confused with Bach1/Fancj/Brip1 helicase.
• There are two BACH1 splice variants with a 24 and 31aa substitution for aa593-736, respectively.
• Over aa 133-513, human BACH1 shares 75% aa identity with mouse BACH1.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.2ug/ml with lysates of K562 human chronic myelogenous leukemia cell line and A172 human glioblastoma cell lineImmunohistochemistry: 5-15ug/ml detects BACH1 in immersion fixed paraffin-embedded sections of human lung cancer tissueOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.