Bak (BAK1, BCL2-antagonist/killer 1, BAK, CDN1, BCL2L7, cell death inhibitor 1, apoptosis regulator BAK, Bcl-2 homologous antagonist/killer) is a 25-30kD member of the BCL-2 family of proteins.
It is widely expressed, and participates in the apoptotic cycle.
BAK is an outer mitochondrial membrane protein that is inactive as a Zn-dependent homodimer.
Upon activation by p53 or tBID, BAK oligomerizes, creating a pore in the mitochondrial membrane and allowing for cytochrome C release.
Human BAK is 211 aa in length and contains three BCL-2 homology domains (aa74-88, 117-136 and 169-184), a Zn-binding region (aa160-166) and a C-terminal transmembrane segment (aa188-205).
Amino acids 67-94 mediate oligomerization of BAK.
There are two potential isoform variants; one shows an alternate start site at Met96, while a second shows a deletion of aa46-66.
Over amino acids 20-124, human BAK shares 76% aa identity with mouse BAK.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity：Recognizes human BAK at ~29kD. Species Cross Reactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant human BAK, aa20-124; Q16611 (E. coli).
