Angiogensin I Converting Enzyme-2 (ACE-2) is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides.
The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function.
ACE-2 isoforms of 75kD and 120kD are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage.
ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus.
Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml using recombinant human ACE‑2Immunohistochemistry: 5-15ug/ml in immersion fixed paraffin-embedded sections of human kidneyOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 2.5mg BSA. Labeled with Biotin. Reconstitute with 1ml sterile PBS.
Specificity：Recognizes human ACE-2 ectodomain. Less than 1% cross-reactivity with recombinant human ACE.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln18-Ser740 of human ACE-2, expressed in NS0 cells.
