Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Flow cytometry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml using human, mouse and rat kidney tissueFlow Cytometry: 0.25ug/10e6 cells using HEK293 human cell line transfected with human ACE2 and eGFPImmunohistochemistry (Paraffin): 3-15ug/ml using fixed, frozen sectiond of rat lung, immersion fixed paraffin-embedded sections of human kidney, hamster lung, rat lung and rat kidneyImmunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human Angiotensin Converting Enzyme 2
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Angiotensin Converting Enzyme 2 ectodomain. Crossreactivity: Recognizes human, mouse and rat ACE2 in Western Blot. Detects hamster ACE2 in Immunohistochemistry. <1% with recombinant human Angiotensin Converting Enzyme
Isotype：IgG
Calc Applications Abbrev：E FC IHC IP WB
Calc Crossreactivity：Hm Hu Mo Rt
Immunogen：Recombinant protein corresponding to Gln18-Ser740 of human Angiotensin Converting Enzyme 2 ectodomain, expressed in NS0 cells
