Angiocidin (also S5a, RPN10a and Mcb1) is a 41kD (predicted) member of the proteosome subunit S5A family of proteins.
It runs anomalously in SDS-page at 55kD.
Intracellularly, Angiocidin is a component of the 26S proteosome that degrades ubiquitinated proteins.
Extracellularly, it inhibits angiogenesis by binding to collagen and beta 1 integrins.
Mouse Angiocidin is 376aa in length.
It contains a vWF-A domain (aa5-188), a beta 1 interacting site (aa86-105) and two ubiquitin-interacting motifs (aa211-230 and 282-301).
There are four splice variants.
Three show a GlyGluArg insertion after Glu254, with two of these showing an additional three aa substitution for aa255-376, and a 45aa substitution for aa321-376, respectfully.
A fourth shows a 51aa substitution for aa299-376.
Over aa1-254, mouse Angiocidin shares > 99% aa identity with rat and human Angiocidin.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1.0ug/mlWestern Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Angiocidin.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：E. coli-derived recombinant protein corresponding to Val2-Glu254 of mouse S5a/Angiocidin isoform A.
