X11-γ (Adaptor protein X11 gamma; also APBA3, Mint3 and X11L2) is a member of the X11 family of adaptor proteins.
Unlike X11-α and -β which are generally neuronal proteins, X11-γ is widely expressed in all tissues.
X11-γ has many functions, including MT-MMP and Furin intracellular trafficking and APP binding.
Human X11-γ is 575 amino acids (aa) in length.
It contains a poly-Ser segment (aa165-171), a PID region (aa217-381) that binds β-amyloid precursor, and two PDZ domains (aa393-478 and 485-560) that bind Arf GTPases.
There is one potential splice variant that shows a 34 aa insertion after Ile505.
Over aa1-138, human X11-γ shares 62% aa identity with mouse X11-γ.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml detects APBA3 in lysates of HeLa human cervical epithelial carcinoma cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul PBS.
Specificity：Recognizes human APBA3. Less than 1% cross‑reactivity with recombinant human (rh) X11‑ alpha and rhX11-beta
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Leu138 from human APBA3, expressed in E. coli.
