84-1111-36　AMICA (Adhesion Molecule that Interacts with CXADR Antigen 1, Junction Adhesion Molecule-Like, JAM-L, JAML) 100ug　A1372-31H4

特徴

• AMICA (adhesion molecule, interacting with CXADR antigen 1), also known as JAML, is a 65kD, heavily glycosylated transmembrane protein that belongs to the junctional adhesion molecule (JAM) subset of the immunoglobulin superfamily.
• JAM family molecules contribute to intercellular connections within epithelial and endothelial cell layers, and mediate their interactions with various hemopoietic cells.
• The human AMICA cDNA encodes a 384aa precursor that includes a 19aa signal sequence, a 256aa extracellular domain (ECD) with two Ig­ like domains, a 21aa transmembrane segment, and a 98aa cytoplasmic domain.
• Alternative splicing may generate isoforms with N­ and C­ terminal deletions.
• In contrast to other JAM family proteins, AMICA does not contain a cytoplasmic PDZ­ binding motif.
• Applications:Suitable for use in Flow Cytometry, ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Flow Cytometry: 2.5/10e6 using Human peripheral blood monocytes
• ELISA (Capture): 0.2-0.8ug/ml can be used as a capture reagent in a human AMICA sandwich immunoassay in combination with the biotinylated human AMICA detection antibody (A1372-31H5) and recombinant human AMICA as the standard.
• Shows <0.3% crossreactivity with recombinant mouse AMICA and recombinant human JAM-A, JAM-B and JAM-C
• Western Blot: 0.1ug/ml using Recombinant Human AMICA/JAML Fc Chimera Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.