Produced in goats immunized with purified, insect cell line Sf 21-derived, recombinant human Agouti-Related Protein (rhAgRP), also known as the protein product of Agouti-related transcript (ART).
AgRP specific IgG was purified by human AgRP affinity chromatography and then biotinylated.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: Can be used as a detection antibody in a human AgRP ELISA in combination with the monoclonal capture antibody (Catalog# A1059-62G).
Using plates coated with 100ul/well of the capture antibody, in combination with 100ul/well of the detection antibody at 50ng/ml, an ELISA for sample volumes of 100ul can be obtained.
To arrive at the optimal dose range for this ELISA, set up a two-fold dilution series of the protein standard stAgRPing with 4ng/ml.
Western Blot: 0.1-0.2ug/ml.
The detection limit for rhAgRP is approximately 1ng/lane under non-reducing and reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 50ug BSA. Reconstitute with sterile Tris-buffered saline pH 7.3 (20mM Tris base, 150mM sodium chloride) containing 0.1% BSA. If 1ml of buffer is used, the antibody concentration will be 50ug/ml.
Specificity：Recognizes human AgRP. ~40% cross-reactivity with rmAgRP.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Sf 21-derived, recombinant human Agouti-Related Protein.
