As the key component of the cartilage extracellular matrix, aggrecan hydrates the collagen network and provides cartilage with its properties of compressibility and elasticity.
Maintenance of aggrecan content is therefore critical to the function of the tissue and aggrecan degradation is an important factor in the erosion of articular cartilage in arthritic diseases.
The deduced amino acid sequence of human aggrecan core protein consists of 2415 resides and predicts a signal peptide and domains of G1, IGD, G2, KS, CS-1, CS-2, and G3.
Two globular domains, G1 and G2, comprise the Nterminus of the proteoglycan and also contain link domains.
The third globular domain, G3, corresponds to the C-terminus.
The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains are between G2 and G3.
With KS and CS attached to the 250kD core protein, aggrecan monomers exist as a 1000-2000 kD molecule.
In addition, aggrecan monomers interact with hyaluronan through their G1 domain, resulting in larger aggregates containing 10-100 aggrecan monomers on a hyaluronan backbone.
Applications:Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 5-15ug/ml in immersion fixed paraffin-embedded sections of human catilage sectionsImmunocytochemistry: 5-15ug/ml in immersion fixed rat mesenchymal stem cells differentiated into chondrocytesWestern Blot: 0.1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Aggrecan G1-IGD-G2 Domains.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Val20-Arg675 from human Aggrecan G1-IGD-G2 Domains, expressed in NS0 cells.
