AFAP (actin filament associated protein; also known as AFAP-110) is an adaptor protein that regulates actin filament integrity.
It is both multimodular and multimeric, and is known to simultaneously bind actin and cytosolic kinases.
As a homomultimer, AFAP binds and separates actin filaments, preventing actin cross-linking.
Upon binding to kinases, AFAP is destabilized, promoting actin polymerization.
Human AFAP is 730aa in length, and contains SH3 and SH2 binding motifs (aa69-74 and aa91-96), two pleckstrin homology domains (aa153-248 and aa347-450) and a Leu-zipper/actin binding region (aa511-637).
Over aa450-730, human AFAP is 86% identical to mouse and rat AFAP.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity：Recognizes human AFAP.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human AFAP aa450-730 (E. coli).
