Human ADPN (Adiponutrin; also PNPLA3 and patatin-like phospholipase domain-containing protein 3) is a 53kD member of the PNPLA family of phospholipase A2 enzymes.
It is expressed in white adipocytes.
Adiponutrin is elevated in response to insulin and appears to act not as a phospholipase, but a molecule involved in anabolic pathways.
ADPN is likely to be a type II transmembrane protein that is 481aa in length.
It contains one patatin domain (aa10-179) that is associated with in vitro enzyme activity and a transmembrane segment between aa42-62.
There appear to be two splice variants.
One shows a four aa deletion of aa59-62 and a second shows an alternate start site at Met379.
Over aa 160-349, human ADPN shares 61% aa identity with mouse Adiponutrin.
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1.0ug/mlWestern Blot: 1.0ug/mlImmunohistochemistry: 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 500ul PBS.
Specificity：Recognizes human ADPN.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived recombinant protein corresponding to Arg160-Arg349 of human Adiponutrin/PNPLA3.
