Applications:Suitable for use in Direct ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml detects both recombinant and natural human ADAMTS1Immunoprecipitation: 25ug/ml detects human ADAMTS1i using conditioned cell culture medium spoke with rhDAMTS1Immunohistochemistry: 5-15ug/ml detects human ADAMTS1 in human tissuesOptimal dilution determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography
Form:Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity:Recognizes human ADAMTS1 Propeptide
Isotype:IgG
Calc Applications Abbrev:E IHC IP WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to Leu50-Arg252 from human ADAMTS1 expressed in NS0 cells.