84-1111-24　ADAMTS5 (Aggrecanase 2, A Disintegrin And Metalloproteinase with Thrombospondin-3 motif) 100ug　A0859-61E4

特徴

• ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5), also known as aggrecanase-2 and ADAMTS11, is a member of the family of secreted zinc proteases with a multi-domain structure (1, 2).
• The protein precursors consist of signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, spacer and a variable number of TS type 1 motifs.
• ADAMTS5 is an active protease effectively cleaving alpha 2-macroglobulin (3), aggrecan (4), and brevican (5), and is inhibited by TIMP-3 with inhibition constants in the subnanomolar range (6).
• Based on the murine model studies (7, 8), this protease may be a key enzyme in the degradation of cartilage leading to osteoarthritis and recombinant human eumatoid arthritis.
• The purified recombinant human ADAMTS5 starts at theN‑terminus of the catalytic domain and ends at the C-terminus of the TSP-1 domain.
• The amino acid sequence of recombinant human ADAMTS5 is 98%, 97%, and 96% identical to that of canine, bovine, and mouse/rat.
• The aggrecanase activity can be inhibited by 5 mM 1,10-phenanthroline and recombinant human TIMP-3.
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Immunoprecipitation: 25ug/ml using conditioned culture medium spiked with recombinant human ADAMTS5
• Western Blot: 0.1ug/ml using recombinant human ADAMTS5 as sampleOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.