ADAMTS4, also known as aggrecanase-1, is a secreted member of the ADAM family of proteins.
ADAMTS4 is upregulated by TNF-alpha in osteoarthritis and contributes to joint destruction.
Its upregulation in some cancers may enhance tumor invasiveness.
Human ADAMTS4 consists of a signal peptide (aa1-51), a pro domain (aa52-212) and a mature chain (aa213-837) containing the following domains: catalytic (aa218-425), disintegrin (aa437-519), TSP type-1 (aa520-575), Cys-rich (aa 577 to 685) and spacer (aa686-837).
The amino acid sequence of rhADAMTS4 is 100%, 97% and 94% identical to that of chimpanzee, dog, and mouse/rat/bovine.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml.
Recombinant Human ADAMTS4Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile TBS, 0.1% BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.5% BSA. Reconstitute with 1ml sterile TBS, 0.1% BSA. Labeled with Biotin.
Specificity：Recognizes human ADAMTS4.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Phe213-Cys685 from human ADAMTS4, expressed in CHO cells. Uniprot #: O75173
