84-1111-22　ADAM 32 (ADAM metallopeptidase domain 32, ADAM 32 precursor, A disintegrin and metalloprotease domain 32, A disintegrin and metalloproteinase domain 32, FLJ26299, FLJ29004, UNQ5982/PRO21340) 100ug　A0859-41M

特徴

• ADAM32 (a disintegrin and metalloprotease domain 32) is a 90-100kD member of the M12B peptidase family of proteins.
• It is expressed on sperm found in the testis, epididymis and vas deferens.
• The human ADAM32 proprecursor is a 771aa type I transmembrane protein.
• It contains a 158 aa proregion (aa17-174) and a 508aa extracellular domain (ECD) (aa175-682).
• The ECD contains a nonfunctional metalloprotease domain (aa186-383), an integrin-binding disintegrin region (aa391-479), a Cys-rich segment (aa480-502) and an EGF-like domain (aa 622-654).
• In the testis, mature ADAM32 is approximately 98kD in size; in the epididymis, cleavage occurs after the metalloprotease domain to generate a 44kD product.
• There are two potential splice events that show a deletion of aa306-401 plus a 53aa substitution for the N-terminal 46aa.
• Over aa17-476, human ADAM32 is 66% aa identical to mouse ADAM32.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1.0ug/mlWestern Blot: 1.0ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile 40-50% glycerol, PBS..
• Aliquot and store at -20°C.
• Reconstituted product is stable for 6 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.