ADAM23 (a disintegrin and metalloprotease domain 23; also MDC3) is a 100kD member of the M12B peptidase family of enzymes.
It is expressed on fetal neurons in the hippocampus and cerebellum and serves as a counter-receptor for alpha v beta 3 integrin.
The human ADAM23 proprecursor is a 773aa type I transmembrane (TM) protein.
It contains a 227aa cleavable proregion (aa60-286) and a 506aa extracellular domain (ECD) (aa287-792) that is part of a 70kD mature molecule.
The ECD contains a nonfunctional metalloprotease domain (aa299- 494), an integrin-binding disintegrin region (aa511-585) and a Cys-rich domain (aa589-611).
Two splice variants exist.
One shows an in-frame 46aa substitution for aa787-832 that generates a soluble form, while a second shows an in-frame 31aa TM substitution for aa787-817.
Over aa 1-585, human ADAM23 is 92% aa identical to mouse ADAM23.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml, using recombinant human ADAM23Immunoprecipitation: 25ug/ml on conditioned cell culture medium spiked with recombinant human ADAM23Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human ADAM23. Less than 5% cross-reactivity with recombinant human ADAM8, recombinant human ADAM9, and recombinant human ADAM22 is observed.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser60-His585 from human ADAM23, expressed in CHO cells.
