ADAM12 (A disintegrin and metalloprotease domain 12; also meltrin-alpha) is a 120kD type I transmembrane glycoprotein member of the metzincin family of proteases.
It is synthesized as a 909aa preproprecursor that contains a 25kD proregion (aa29-207) and a 92kD mature molecule.
Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface expression.
A 68kD soluble splice form exists in pregnancy where 36aa replace the C-terminal 205aa (705-909).
This circulating ADAM12 isoform remains associated with its prodomain via noncovalent linkage, possibly acting as an enzyme regulator.
Over aa29-513, human ADAM12 shares 78% and 79% aa identity with bovine and mouse ADAM12, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile 40-50% glycerol, PBS..
Aliquot and store at -20°C.
Reconstituted product is stable for 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with BSA. Reconstitute with 40-50% glycerol, PBS.
Specificity：Recognizes human ADAM12.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：CHO cell-derived, recombinant human ADAM12 aa29-513; O43184; Glu36Gln substitution.
