ADAM12 (A disintegrin and metalloprotease domain 12; also meltrin-α) is a 120kD type I transmembrane glycoprotein member of the metzincin family of proteases.
It is synthesized as a 909 amino acid (aa) preproprecursor that contains a 25kD proregion (aa 29-207) and a 92kD mature molecule.
Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface expression.
A 68kD soluble splice form exists in pregnancy where 36 aa replace the C-terminal 205 aa (705-909).
This circulating ADAM12 isoform remains associated with its prodomain via noncovalent linkage, possibly acting as an enzyme regulator.
Over aa 29-513, human ADAM12 shares 78% and 79% aa identity with bovine and mouse ADAM12, respectively.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Immunoprecipitation: 25ug/mlWestern Blot: 0.1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, pH 7.4, with 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human ADAM12.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa29-513 with a Glu36Gln substitution human ADAM12 expressed CHO cell.
