84-1111-06　Activin Receptor Type IIB (RIIB) 100ug　A0856-10A

特徴

• Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction.
• Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11.
• Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated.
• Applications:Suitable for use in Neutralization, ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Neutralization: ~1-3ug/ml will block 50% of the binding of 30ng/ml of recombinant human Activin A to immobilized recombinant human Act RIIB/Fc Chimera (100ul of a 2ug/ml solution was coated in each well) in an ELISA type assay
• Direct ELISA: 0.5-1.0ug/ml.
• The detection limit is ~0.1ng/well
• Western Blot: 0.1-0.2ug/ml.
• The detection limit is ~1ng/lane under non-reducing and reducing conditions
• Immunohistochemistry (Paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.