84-1110-99　Activin Receptor Type IB (ALK-4, RIB) [KO Validated] 100ug　A0855-90C

特徴

• Applications:Suitable for use in Neutralization, ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.1ug/ml using lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, or HEK293T human embryonic kidney cell lineNeutralization of receptor-ligand interaction: 0.5-2ug/ml Blocks 50% of the binding of 50ng/mL of rhCripto 1 to immobilized rhActRIB/Fc (100ul of 2ug/ml was coated in each well) in a functional ELISA.
• At 20ug/ml there will be >90% blockingKO Validation: Activin RIB/ALK‑4 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Activin RIB/ALK‑4 knockout HeLa cell line
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.