Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily.
Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C.
The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain.
This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively.
A33 is likely to be involved in cell-cell adhesion between epithelial cells.
Applications:Suitable for use in Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 0.25ug/10e6 cells using human HT-29 human colon carcinoma cell line
Western Blot: 0.1ug/ml using recombinant human A3Optimal dilutions to be determined by the researche
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 50ug BSA. Reconstitute with 250ul sterile PBS.
Specificity：Recognizes human A33. Species Crossreactivity: ~10% crossreactivity with recombinant mouse A33.
Isotype：IgG
Calc Applications Abbrev：FC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to lle22-Val235 of human A33 extracellular domain, expressed in NS0 cells.
