Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily.
Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C.
The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain.
Applications:Suitable for use in Flow Cytometry, Direct ELISA, Western Blot and Immunohistochemistry.
Tested using TH-29 human colon carcinoma cell lineWestern Blot: 0.1ug/ml using recombinat human A33Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human colonOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human A33. Crossreacts with recombinant mouse A33 ~10%.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ile22-Val235 from human A33 expressed in NS0 cells.
