4-1BB, also known as CD137, is a type I transmembrane protein belonging to the TNF receptor superfamily (TNFRSF9).
It is expressed on T cells, NK cells, monocytes, neutrophils, dendritic cells, and eosinophils.
The ligand for 4-1BB, 4-1BBL, is a TNF ligand superfamily member (TNFSF9) expressed on the surfaces of activated antigen presenting cells and T and B lymphoma cell lines.
4-1BB transduces a T cell costimulatory signal to promote survival, proliferation, cytokine production, and effector functions.
On dendritic cells, 4-1BB ligation enhances cytokine production and upregulates expression of B7-1 and B7-2 costimulatory molecules.
Applications:Suitable for use in RIA, Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 3-10ug/ml/10ELISA: 0.5-1.0ug/mlWestern Blot: 0.1-0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2 um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.5ml of PBS is used, the antibody concentration will be 0.2mg/ml.
Specificity：Recognizes 4-1BB Ligand/TNFSF9.
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human 4-1BB Ligand aa71-254 (E. coli).
