AUP1 contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain thay may be involved in binding ubiquitin-conjugating enzymes.
The unprocessed precusor is of 476 amino acids in length and has an estimated molecular weight of 53kD.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry (paraffin embedded and frozen tissues):1:500 -1:6000.
Western Blot: 1:500 -1:6000
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile 40-50% glycerol, ddH2O.
Reconstituted product is stable for 12 months at -20°C.
Aliquot and store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ul
Host:sheep
Source Antibody:human
Grade:Serum
Purity:Serum
Form:Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.
Specificity:Recognizes ancient ubiquitous protein 1. Species crossreactivity: bovine, sheep, human and rat.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Bo Hu Rt Sh
Immunogen:A synthetic peptide (HVFLVSCALPDSV) corresponding to the aa48-60 of human ancient ubiquitous protein 1 conjugated to blue carrier protein.