TrkA is a member of the neurotrophic tyrosine kinase receptor family.
It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway.
TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF).
TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes.
It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating.
Subunit: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:ELISA: 1:5000Immunohistochemistry: 1:1000Western Blot: 1:1000-1:2500 under denaturing and non-reducing conditionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human TrkA. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Extracellular domain of glycosylated human TrkA protein produced in CHO cells.
