Tau protein promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity.
The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both.
Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome.
The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Tau is expressed in neurons.
Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.
The tau/MAP repeat binds to tubulin.
In Alzheimer’s disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments and straight filaments, mainly composed of hyperphosphorylated forms of Tau.
Defects in Microtubule-associated protein Tau are a cause of frontotemporal dementia and parkinsonism linked to chromosome 17, as well as a number of other neurodegenerative diseases.
Applications:Suitable for use in Western Blot and ELISA (Direct).
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human Microtubule-associated Tau proteins. Detects the purified recombinant Tau expressed in E. coli as well as a number of Tau isoforms in porcine cytosol. Species Crossreactivity: porcine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Po
Immunogen：Recombinant human Microtubule-associated protein Tau, purified from E. coli. Cellular Localization: Cytoplasm; cytosol. Cell membrane. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
