Human zona occludens 2 (ZO-2) is an ~160kD tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family.
Members of this family are involved in epithelial and endothelial intercellular junctions.
They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain.
PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners.
PDZ domain-containing proteins, like ZO-2, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact.
The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes.
ZO-2 can interact with zona occludens 1 (ZO-1).
Furthermore, the PDZ2 domain of ZO-2 was shown to interact with connexin-43, the predominant connexin in epithelial and most other tissues which is involved in cell growth control and embryonic development.
Applications:Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50Western Blot: 1:50Immunohistochemistry (frozen): 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：guinea pig
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity：Recognizes human zona occludens 2 (ZO-2). Species crossreactivity: mouse
