84-1110-39　ZONA OCCLUDENS 1 alpha-minus (ZO-1 alpha-minus) 100ug　Z0750-11A

特徴

• Human zona occludens 1 (ZO-1) is an ~220kD tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family.
• Members of this family are involved in epithelial and endothelial intercellular junctions.
• They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain.
• PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners.
• PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact.
• The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes.
• ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties.
• ZO-1 immunoprecipitates with its family member ZO-2.
• ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling.
• Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene.
• The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus.
• The alpha-containing isoform is found in most epithelial cell junctions.
• The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
• This difference in distribution provides molecular distinction among tight junctions.
• Applications:Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:50Western Blot: 1:50Immunohistochemistry (frozen and paraffin): 1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.