84-1110-36　Surfactant Protein B (SP-B) 100ug　S8401-02D

特徴

• There are four surfactant-specific proteins, designated surfactant protein A (SP-A), SP-B, SP-C and SP-D respectively.
• SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family.
• SP-B and SP-C are hydrophobic surfactant proteins and may be the most appropriate indicators for the evolutionary origin of surfactant.
• SP-B is synthesized by the alveolar type II epithelial cells as a 40-42 kD precursor that is subsequently proteolytically processed to 7.8-8 kD.
• SP-B enhances the spreading and stability of surfactant phospholipids in the alveolus.
• SP-B is essential for air-breathing in mammals and is therefore largely conserved.
• SP-B can interact with both phospholipid head groups and fatty chains and is particularly active in enhancing surface active behavior in endogenous and exogenous lung surfactants.
• Even low SP-B contents had measurable effects in increasing the adsorption, dynamic surface tension lowering, and/or film respreading of DPPC, mixed synthetic lipids, and column-purified lung surfactant phospholipids.
• Deficiency of SP-B and other surfactant components is associated with respiratory distress syndrome (RDS) in premature infants and adults with respiratory distress syndrome (ARDS).
• Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:50.
• Recognizes proteins of 42, 25 and 18kD under non-reducing conditions.
• The 18kD form is reduced to 7.7-8kD in the presence of beta-mercaptoethanol
• Immunohistochemistry (Frozen, paraffin): 1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.