Human indoleamine 2,3-dioxygenase (IDO) is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules, like tryptophan, serotonin and melatonin.
By doing this, IDO initiates the production of biologically active metabolites, commonly referred to as kynurenines.
IDO is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs).
In inflammation, interferons (IFNs) act on specific receptors to trigger IDO induction.
The production of INF-gamma and induction of IDO represent important antimicrobial mechanisms.
Degradation and depletion of tryptophan by IDO inhibits the growth of viruses, bacteria and parasites.
Furthermore, IDO plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.
By local depletion of tryptophan and increasing proapoptotic kynurenines, IDO greatly affects T-cell proliferation and survival, both in vitro and in vivo, and also affects B-cell and NK-cell function and survival.
There is a central role for IDO expression in tolerance involving regulatory cells and DCs.
IDO acts as an intermediate pathway in LPS-induced production of reactive oxygen species and NF-kappaB activation, two processes that lead to DC maturation.
Applications:Suitable for use in ELISA, Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:50; Detection antibodyImmunofluorescence: 1:50Flow Cytometry: 1:50; It’s recommended that this antibody be used in combination with fixing and permeabilization
Western Blot: 1:50Immunohistochemistry (Paraffin): 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity：Recognizes human indoleamine 2,3-dioxygenase (IDO). Reactivity with IDO has been confirmed on Western Blot with IFN-gamma stimulated human peripheral blood lymphocytes.
Isotype：IgG
Calc Applications Abbrev：E FC IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human IDO.
