84-1110-27　Pyruvate Dehydrogenase E1-alpha Subunit, phosphorylated (Ser293) (Pyruvate Dehydrogenase E1 alpha, Pyruvate Dehydrogenase E1 Component Subunit alpha Somatic Form Mitochondrial, PDH, PDHCE1A, PDHE1-A Type I, PHE1A, Pyruvate Dehydrogenase (Lipoamide) alpha 1, PDHA1) 50ul　P9544-15

特徴

• The pyruvate dehydrogenase complex (PDC) is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA.
• The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1 or PDHE1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD).
• The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits.
• The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex.
• Enhanced expression of pyruvate dehydrogenase kinase-1 (PDK-1) results in phosphorylation of PDHE1 alpha at Serine 293 which in turn produces nearly complete inhibition of the pyruvate dehydrogenase complex (PDC).
• A recent study shows that inhibition of PDC activity in cancer cells restores normoxic stablization of HIF-1 alpha by glycolytic metabolites.
• Applications:Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000-1:5000 detects a band at ~43kD
• Immunofluorescence (IC): 1:50-1:250Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.