N-Acylphosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a membrane-bound enzyme which releases the endocannabinoid anandamide and other bioactive N-acylethanolamines from their corresponding NAPEs in animal tissues.
NAPE-PLD has been shown to be expressed by specific populations of neurons in the brain and targeted to axonal processes.
Recent studies have suggested that NAEs generated by NAPE-PLD in axons may act as anterograde synaptic signaling molecules that regulate the activity of postsynaptic neurons.
NAPE-PLD has also been shown to catalyze the hydrolysis of NAPE to generate OEA (oleoylethanolamide), a natural lipid amide that inhibits food intake in free-feeding rodents by prolonging latency to feed and postmeal interval.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Immunofluorescence (IC): 1:1000-1:5000Western Blot: 1:2500-1:5000Optimal dilutions to be determined by the researcher.
Positive Control: Mouse Brain Whole Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.09% sodium azide
Specificity：Recognizes mouse NAPE-PLD at ~50kD. Species Crossreactivity: human, rat and fungi
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa1-100 from the internal portion of mouse NAPE-PLD
