All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure.
The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix.
Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions.
MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM.
MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion.
These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases).
TIMPs have been shown to block tumor cell invasion suggesting that they act as metastasis suppressor genes.
MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes.
The expression of MMP9 increases in Epstein-Barr virus infected lymphoma derived cell lines and may be of significance in typically invasive nasopharyngeal carcinomas.
MMP9 is constitutively produced by some tumor cell lines (e.g.: HT1080, HL60, U937) but not by most quiescent cells and tissues.
Treatment of cells with the phorbol ester TPA stimulates production of MMP9 in some cell types, but the low protein levels produced (pg/ml) often require concentration of cell culture media to visualize the bands by Western blotting.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 3 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
