84-1110-10　Hypoxia Inducible Factor 1 alpha (Hypoxia Inducible Factor 1 alpha Subunit Basic Helix-loop-helix Transcription Factor, HIF1A, HIF-1 alpha, HIF-1A, HIF1-alpha, ARNT Interacting Protein, Basic Helix-loop-helix PAS Protein MOP1, Member of PAS Protein 1, Member of PAS Superfamily 1, Member of the PAS Superfamily 1, MOP1, PASD8) (Biotin) 100ul　H9804-04J

特徴

• Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
• HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis.
• This occurs as a posttranslational modification by prolyl hydroxylation.
• HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits.
• Both subunits are constantly translated.
• However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes.
• Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity.
• Therefore, the concentration of HIF-1 alpha increases in the cell.
• In contrast, HIF-1 beta remains stable under either condition.
• HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
• Applications:Suitable for Western Blot and Immunhistochemistry.
• Not recommended for Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:2000Immunohistochemistry (Frozen/paraffin): 5-10ug/mlOptimal dilutions to be determined by researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.