84-1110-08　Hypoxia Inducible Factor 1 alpha (Hypoxia Inducible Factor 1 alpha Subunit Basic Helix-loop-helix Transcription Factor, HIF1A, HIF-1 alpha, HIF-1A, HIF1-alpha, ARNT Interacting Protein, Basic Helix-loop-helix PAS Protein MOP1, Member of PAS Protein 1, Member of PAS Superfamily 1, Member of the PAS Superfamily 1, MOP1, PASD8) 100ul　H9804-04G

特徴

• Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
• HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis.
• This occurs as a posttranslational modification by prolyl hydroxylation.
• HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits.
• Both subunits are constantly translated.
• However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes.
• Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity.
• Therefore, the concentration of HIF-1 alpha increases in the cell.
• In contrast, HIF-1 beta remains stable under either condition.
• HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Positive Control: COS-7 nuclear extracts (treated)
• Cellular Localization: Nuclear
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
• Aliquots are stable for at least 3 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.