Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.
Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules.
Several molecules including caspases and adaptor FADD contain DEDs.
A novel protein that interacts with DED of caspase-8 and –10, and FADD was identified recently and designated DEDAF for DED associated factor (1).
DEDAF is identical to the transcriptional repressor RYBP (2).
DEDAF/RYBP is expressed in multiple tissues and cell lines.
DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1 ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human A549 or HepG2 cell lysate
Storage and Stability:May be stored at 4°C.
For long-term storage, aliquot and store at 4°C.
Do not freeze.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human DEDAF at ~32kD. Species Crossreactivity: Mouse and rat. The sequence is identical to that of mouse origin (2).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 215-228 of human DEDAF (1,2).
